2월 '의약화학 항암제' 공식 출시 2020 푸젠성 의과대학 약학부 리펑 교수팀 연구결과 발표. The research confirmed through cell and animal experiments that the neutral triterpenes from영지버섯 can significantly inhibit the growth of colorectal cancer, and its mechanism of action is related to “promoting cancer cell apoptosis.”
Total Triterpenes = Neutral Triterpenes + Acidic Triterpenes
Since the first discovery of영지버섯 triterpenes in 1982, scientists have not only provided scientific explanations for “why영지버섯 fruiting bodies are so bitter” but also provided clues other than polysaccharides for the study of “Why영지버섯 is anti-tumor”.
영지버섯 triterpenes are a collective noun, which refers to the active ingredients in영지버섯 with terpene structure. According to their chemical structure, they can be divided into two groups: one group is “acidic triterpenes” including various ganoderic acids (acidic triterpene fraction, ATF), and the other group is “neutral triterpenes” including various ganoderiols (“neutral triterpene fraction”, NTF). When these two groups of triterpenes are combined, they are called total triterpenes.
Although there are many scientific evidences for the anti-tumor effects of영지버섯 total triterpenes and acidic triterpenes, there are few studies on the role of영지버섯 neutral triterpenes in this regard. 그러므로, Professor Li Peng’s team focused on this part.
Using영지버섯 자실체 (Fujian Xianzhilou Biological Science and Technology Co. 제공, 주식회사) as the experimental material, the team first extracted the total triterpenes of영지버섯 자실체에서영지버섯 with ethanol, and then further separated the neutral triterpenes and acidic triterpenes to explore their inhibitory effect on colorectal cancer.
Cell experiment: the anti-cancer effect of neutral triterpenes＞the anti-cancer effect of acidic triterpenes
The researchers separately cultured영지버섯 neutral triterpenes and acidic triterpenes with three different types of human colorectal cancer cells for 48 시간. 전체적으로, 영지버섯 neutral triterpenes had significantly better inhibitory effects on cancer cell growth (proliferation) than영지버섯 acidic triterpenes (수치 1).
Animal experiment: 영지버섯 neutral triterpenes effectively inhibit tumor growth
The researchers further evaluated the anti-tumor effects of영지버섯 neutral triterpenes in vivo through animal experiments: 첫 번째, the human colorectal cancer cell line SW620 with lymphatic metastasis ability was implanted under the skin of nude mice (immune-deficient mice). After the tumor has emerged, 250 mg/kg 또는 500 mg/kg of영지버섯 neutral triterpenes are given orally to the mice every day.
후에 13 days of experiments, it was found that the intervention of영지버섯 neutral triterpenes can make tumors grow slower and smaller, and its inhibitory effect is comparable to that of the chemotherapy drug 5-Fu (20 mg/kg intraperitoneal injection per day), but it doesn’t cause serious weight loss like 5-Fu (수치 2-5).
Active ingredients: at least nine kinds of triterpenes
As mentioned earlier in the article, 영지버섯 neutral triterpenes are also a mixture, which contains a variety of different triterpenes. 연구원의 분석에 따르면, the above-mentioned neutral triterpenes of영지버섯, which has an inhibitory effect on colorectal cancer, contain at least nine kinds of triterpenes (수치 6).
Mechanism of action: to promote apoptosis of cancer cells
If these nine triterpenes are cultured separately with the human colorectal cancer cell line SW620 in vitro, they will be found to be more or less capable of killing cancer cells.
The researchers further explored the most representative ganoderma triterpene (가노데르마논디올) and found that it can initiate the apoptosis mechanism of cancer cells and push cancer cells from the endless altar to the abyss of death. Its effective concentration (inhibition of half of cancer cells) ~이다 11.17 μg/mL.
This concentration has no lethality on normal cells (mouse embryonic fibroblast cell line NIH3T3), and the concentration must be increased to more than 80 μg/mL to have a significant effect (inhibit the survival of half of mouse embryonic cell lines). This means that ganodermanondiol can distinguish cancer cells from normal cells and give different “treatments”, which is completely different from the “all killing” effect of chemotherapeutic drugs on good and bad cells.
Whether these영지버섯 neutral triterpenes initiate the apoptosis mechanism of cancer cells is achieved by regulating the mitochondria in cancer cells is worthy of further investigation according to the researchers.
[참고자료]
Li P, 외. Anti-Cancer Effects of a Neutral Triterpene Fraction from Ganoderma lucidum and its Active Constituents on SW620 Human Colorectal Cancer Cells. Anticancer Agents Med Chem. 2020; 20(2): 237-244. 도이: 10.2174/1871520619666191015102442.
끝
저자 소개 / 씨. 우팅야오
Wu Tingyao has been reporting on first-hand Ganodermainformation since 1999. 그녀는 다음의 저자입니다.Ganoderma로 치유 (4월 인민의학출판사에 게재 2017).
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